Journal: Cureus
Article Title: An In Vitro Investigation of Levofloxacin-Induced Cytotoxicity in Rat Bone Marrow Mesenchymal Stem Cells
doi: 10.7759/cureus.77802
Figure Lengend Snippet: A-E: RT-PCR mRNA expression of MMP-3, MMP-13, TIMP-1, TIMP-3, and COL1A1 after Levofloxacin therapy at various dosages. The findings were expressed as a ratio relative to the control (β-Actin). Every strip illustrates the mean ± SD (n = 6/group). *p < 0.05; **p < 0.01 BMSC: bone marrow mesenchymal stem cell; RT-PCR: reverse transcriptase polymerase chain reaction; mRNA: messenger RNA; MMP: matrix metalloproteinase; TIMP: tissue inhibitor of metalloproteinase; Col1A1: collagen type I alpha 1
Article Snippet: Reverse transcriptase polymerase chain reaction (RT-PCR) assessment of messenger RNA (mRNA) expression Total RNA was isolated with the aid of TRIzol reagent (Takara, Dalian, China), as per the manufacturer’s protocols.
Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Control, Stripping Membranes, Reverse Transcription, Polymerase Chain Reaction